Bioinformatics Analysis

• Protein sequence alignment
• In silico gene expression analysis (microarray)
• Identification of cis-regulatory elements

Biochemical Analysis

• Protein quantification
• Protein electrophoresis in polyacrylamide gel
• Protein separation by ion-exchange chromatography
• Spectrophotometric determination of enzyme activity

Molecular Analysis Methods

• Isolation and quantification of nucleic acids
• Separation of nucleic acids and amplicons by agarose or polyacrylamide gel electrophoresis
• Qualitative and quantitative analysis of transcripts: RT-PCR, qPCR
• Molecular screening: testing genetically modified plants, marker-assisted selection, gene identification by molecular marker mapping
• PCR-based assays: RAPD, SSR, SCAR, CAPS, AFLP